No, these two potential problems are unrelated
If the EV diaphragm is failed then water will eventually fill the air space and the PRV will lift at 3 bar, to check, just depress the schrader valve pin, if you now get water emitting then the diaphragm has failed but even if no water I would suggest keeping the valve pin depressed until the diaphragm is up against the air end. If no water then just pump the air end up to give the same pressure as at the water end was before you commenced the test.
if OK run the system for a few more weeks with the filling hose disconnected.