I'd prefer to just have the one ICG PRV with a mains pressure of 4.5bar, the PRV is then fully in control to maintain that 3.0bar, it probably has nothing to do with your problem but I just don't think its a good idea to have two PRVs in series at such a low dP of, 4.5-3.0, 1.5bar.
You drained the UVC down last night, when you carried out the same tests after refilling, was the UVC still full of cold water or did you wait until it had fully reheated?