Sounds like you'll have a head tank to keep the system topped up with water? This sounds like it is *not* a sealed (newer) type system.
You'll need to turn off the mains supply to the header tank. I would let it drain down when you partially drain the system rather than try to stop it draining.
Open your drain valve (at the lowest point in your system) and keep an eye on the header tank. Once it is empty, the level will quickly go down to the upstairs rads. Open a bleed screw on one and once you hear air being pulled in, fully unscrew that valve and open bleed screws on the others upstairs (if you have TRVs or shut-off valves on your rads, you could try closing those on all rads except the one you need to remove. Trouble is that if these don't cloe fully, you get a slow leak back into the system and out the pipes you are working on. Hence, might be best to leave valves open and drain all upstairs rads).
You can tap the rads and you'll notice the noise change and you can usually tell when they are empty. When you think they are, shut off your drain valve downstairs and slowly loosen the connector at on end of your rad. You'll probably get a few drips, but if you get a good stream of water, you need to drain the system a bit more.
Eventually, you'll have an empty upstairs system (or just that rad if you decided to close TRVs etc on the others). You can then do the work.
When refilling, double check all connections you have disturbed and close all bleed screws. Re-establish supply to the head tank and once it is full, go around all the rads and open the TRVs then bleed the rads.
Once your happy that you think you have all the air out, make sure all TRVs and valves are open and fire up the boiler. Once at operating temp, you should bleed the rads again.
Check for leaks at all stages!
Job done.
Good luck!