Having spent half an hour on the phone to Wunda this morning, unfortunately there was no conclusion. They basically asked me to do some simple stuff like ensure the return valves were open and adjust the flow valves etc, all of which I've already done, so I wasn't surprised that this made no difference. While helpful, the tech support guy seemed genuinely confused about what could be causing the problem.
The more I think about it the more obvious it seems to be: air trapped in the top bar of the manifold. This is the highest point of the underfloor heating system so is where air is likely to gather. Plus, the top bar never gets warm and the flow gauges have no affect, all suggesting the air is preventing water from moving around.
One interesting thing he said was that it was unusual for the supplied wireless thermostat not to be used with their underfloor heating kits. When fitting the UFH, the plumber decided it wasn't necessary to use another thermostat and so the existing weather compensation thermostat in the hallway regulates both the radiators and the UFH. There is also a thermostat on the manifold. Given that the boiler is constantly changing the CH temperature, I did wonder how well this would work as my understanding is that UFH should ideally be kept at a constant lower temperature and that it takes hours for temperature variations to take effect, unlike radiators, which can be instantly changed.