Much depends on how the transformer is wired. Many have the primary first then secondary but some are wired side by side. So some work but not very well and others work A1.
And it depends what you want to do with the transformers a 300 ohm to 50 ohm impedance matching will work either way so long as frequency is correct.
Are you thinking of Power or matching?
For real transformers ( two or more coils ) the answer is generally yes but there are a lot of points to bear in mind.
In rush current may be much higher than expected. Transient voltages across the high voltage winding may be high enough to damage the winding ( insulation break down ) if there is no load on it when the supply to the low voltage winding is switched off.
It must be AC and the frequency must be the frequency that the transformer is designed for.
The working voltages should not be exceed.
When working backwards the maximum power rating needs to be reduced to 80% or less of the rated power forwards.
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